Characterization of recombinant human growth differentiation factor-9 signaling in ovarian granulosa cells
نویسندگان
چکیده
منابع مشابه
Characterization of recombinant human growth differentiation factor-9 signaling in ovarian granulosa cells.
Growth differentiation factor-9 (GDF9) is an oocyte secreted paracrine factor essential for mammalian ovarian folliculogenesis. Like other members of the transforming growth factor-beta (TGFbeta) superfamily, GDF9 is synthesized as a prepropeptide which needs processing by furin-like proteases to result in an active mature protein. We have previously characterized a preparation of unpurified re...
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Background & Aims: Stem cells are undifferentiated cells and are found in different tissues. These cells have capacity of self-renewal and differentiation into other lineages. Granulosa cells (GCs) are the multipotent stem cells. In the present research we evaluated the differentiation potential of GCs into keratinocytes. Material & Methods: GCs were cultured after enzymatic isolation from ova...
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Ovarian folliculogenesis is driven by the combined action of endocrine cues and paracrine factors. The oocyte secretes powerful mitogens, such as growth differentiation factor 9 (GDF9), that regulate granulosa cell proliferation, metabolism, steroidogenesis and differentiation. This study investigated the role of the epidermal growth factor receptor (EGFR)-extracellular signal-regulated kinase ...
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The modulating actions of peptide growth factors on ovarian follicular maturation were studied in granulosa cells during CAMP-dependent differentiation induced by follicle-stimulating hormone (FSH). During a 48-h culture of granulosa cells from hypophysectomized diethylstilbestrol-treated immature rats, FSH induced a 100-fold rise in cAMP and progesterone accumulation and a Ibfold increase in l...
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Ovarian inhibin production is stimulated by FSH and several TGFbeta family ligands including activins and bone morphogenetic proteins. Growth differentiation factor-9 (GDF-9) derived by the oocyte is a member of the TGFbeta/activin family, and we have previously shown that GDF-9 treatment stimulates ovarian inhibin-alpha content in explants of neonatal ovaries. However, little is known about GD...
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ژورنال
عنوان ژورنال: Molecular and Cellular Endocrinology
سال: 2008
ISSN: 0303-7207
DOI: 10.1016/j.mce.2007.11.007